ROLE OF ASCORBIC ACID IN AMELIORATING ARSENIC INDUCED TOXIC EFFCTS ON PROTEIN AND LIPID CONTENT IN FLESH OF Cyprinus Carpio (LINN.)
Keywords:
Arsenic, Ascorbic acid, Lipid, Protein, FishAbstract
Arsenic in aquatic ecosystems is a rising concern for its deleterious effects on aquatic flora and fauna and eventually humans which are dependent on them as an important food source. In the present study fingerlings of Cyprinus carpio were exposed to arsenic for assessing the toxic effects of arsenic on protein and lipid content of muscles of fish. There were six groups considered viz. negative control, positive control, 1/5thLC50As, 1/10thLC50As, 1/5thLC50As+AA, 1/10thLC50As+AA. Sampling was done after 14 and 28 days. Significant decrease in total lipid and protein content was reported in arsenic contaminated groups when compared with negative control. A significant improvement in the total lipid and protein content of the flesh of fish was recorded at all periods of exposure when compared with their respective arsenic treated groups thus establishing the ameliorating potential of ascorbic acid against arsenic toxicity. A dose and time dependent response of arsenic toxicity was documented in the current study. The present study holds importance in areas where ponds and rivers are highly contaminated with arsenic and in regions where groundwater is also severely contaminated with arsenic.
References
Abdel-Hameid N A H. 2009. Effect of calcium carbonate against arsenic toxicity of the Nile catfish, Clariasgariepinus. Turk J Fish AquatSci 9:191-200. Baldissarelli L A, Capiotti K C, Bogo M R, Ghisleni G and Bonan, C D. 2012. Arsenic alters behavioral parameter and brain ectonucleotidases activities in zebrafish (Danio rerio). Comparative Biochemistry and Physiology Part C: Toxicol Pharmacol 155: 566–72. Bears H, Richards J G and Schulte P M. 2006. Arsenic exposure alters hepatic arsenic species composition and stress-mediated gene expression in the common killifish (Fundulus heteroclitus). Aquat Toxicol 77: 257-66. Begum A, Mustafa A I, Amin M N, Banu N and Chowdhury T R. 2013. Accumulation and histopathological effects of arsenic in tissues ofshinghi fish (stinging catfish) Heteropneustesfossilis (Bloch, 1794).J Asiat Soc Bangladesh, Sci 39(2): 221-30.
Bera A K, Rana T, Das S,Bandyopadhyay S, Bhattacharya D, Pan D, De S and Das S K. 2010. L-Ascorbate protects rat hepatocytes against sodium arseniteinduced cytotoxicity and oxidative damage. Hum Exp Toxicol 29: 103‒11.
Cockell KA, Hilton JW. 1988. Preliminary investigations on the comparative chronic toxicity of four dietary arsenicals to juvenile rainbow trout (Salmo gairdneri R.). Aquat Toxicol 12:73–82.
Das D, Sarkar D, Bhattacharya S. 1998. Lipid peroxidative damage by arsenic intoxication is countered by glutathioneglutathioneS-transferase system and metallothionein in the liver of climbing perch, Anabas testudineus. Biomed Environ Sci 11:187–195.
Edmonds J S and Francesconi K A. 1993. Arsenic in sea foods: human health aspect and regulations. Marine Pollut 26: 665- 674.
Folch J, Lees M, Stanley G H S. 1957. A simple method for isolation and purification of total lipids from animal tissues. J Biol Chem 13: 497-509.
Gonzalez HO, Hu J, Gaworecki KM, Roling JA, Baldwin S, GardeaTorresdey JL, Bain L J. 2010. Dose-responsive gene expression changes in juvenile and adult mummichogs (Fundulus heteroclitus) after arsenic exposure. Mar Environ Res 70(2):133–141.
Hughes M F. 2002. Arsenic toxicity and potential mechanisms of action.Toxicol Lett 133: 1-16.
Jana S and Bondyopadhyay N. 1981. Effect of heavy metals on some biochemical parameters in the freshwater fish, Channa punctatus. Environ Ecol 5(3): 488-93.
Kitchin K T and Ahmad S. 2003. Oxidative stress as a possible mode of action for arsenic carcinogenesis. ToxicolLett137: 3-13.
Lam SH, Wu YL, Vega VB, Miller LD, Spitsbergen J, Tong Y, Zhan H, Govindarajan KR, Lee S, Mathavan S, Krishna Murthy KR, Buhler DR, Liu E T and Gong Z. 2006. Conservation of gene expression signatures between zebrafish and human liver tumors and tumor progression. Nat Biotechnol24:73–75.
Lowry O H, Rosebrough N J, Farr A L and Randall R J. 1951. Protein measurement with the Folin phenol reagent. J Biol Chem 193:265-75.
Maruthanayagam C and Sharmila G. 2004.Biochemical variations induced by monocrotophos in Cyprinus carpio during the exposure and recovery period. Nat Environ Poll Tech 3(1): 1-9.
Minokoshi Y, Saito M, Shimazu T. 1988.Sympathetic activation of lipid synthesis in brown adipose tissue in the rat. J Physiol (Lond) 398: 361–70
Pazhanisamy K and Indra N. 2007. Toxic effects of arsenic on protein content in the fish, Labeorohita (Hamilton). NEPT6(1): 113-16.
Rahman M A, Hasegawa H, Lim R P. 2012.Bioaccumulation, biotransformation and trophic transfer of arsenic in the aquatic food chain. Environment Research116:118-135.
Rajamannar K and Manohar L. 1998.Sublethal toxicity of certain pesticides on carbohydrates, protein and amino acids in Labeo rohita. J Ecobiol 10(3): 185-91.Schlenk D, Wolford L, Chelius M, Stevens J,
Chan KM. 1997. Effect of arsenite, arsenate, and the herbicide monosodium methyl arsonate (MSMA) on hepatic metallothionein xpression and lipid peroxidation in channel catfish. Comp Biochem Physiol C 118:177–183.
Singh S and Rana S V S. 2007. Amelioration of arsenic toxicity by LAscorbic acid in laboratory rat. J Environ Biol 28(2): 377-84.
Thatheyus J A, Selvanayagam M and Sebastin Raja S. 1992. Toxicity of nickel on protein content in tissues of Cyprinus carpio (Linn). ndian J Environ Hlth 34(3): 236-238.
Zhang W, Liu Y, Ge M, Yao C, Xue J, Zhang Z. 2014. Resveratrol reduces oxidative stress and improves arsenic efflux in rats exposed to rsenic trioxide. Pak Vet J34: 251-53