IN VITRO EVALUATION OF THE NEMATICIDAL EFFECT OF SOME MEDICINAL PLANTS ON HAEMONCHUS CONTORTUS (NEMATODA)
Keywords:
Nematoda, anthelmintic, nematicidal, medicinal plants, Haemonchus contortusAbstract
The aim of present study is to investigate the nematicidal efficacies of five medicinal plants, Acacia nilotica, Azadirachta indica, Dalbergia sissoo, Ocimum sanctum and Ficus religiosa. Aqueous extracts of these plants were investigated in vitroconditions for their anthelmintic activity against adults of Haemonchus contotus.Aqueous extract of each plant was used at five concentrations (10, 20, 30, 40 and 50 %). The results showed that all the extracts has a dose related effect on the motility of worms and the mortality of the worms increases as the concentration of the extract increases. The overall findings of the study revealed that aqueous extracts prepared from the medicinal plants studied have potential nematicidal properties and in vivo studies are suggested to make use of these plants as effective drugs against Haemonchosis in small ruminants as an alternative to chemotherapy.
References
Adama, K., Gaston, B.A.M., Tamboura, H.H., Traore, A. and sawadogo, L. (2009) In vitro anthelmintic effect of two medicinal plants (Anogeissus leiocarpus and Daniellia oliveri) on Haemonchus contortus, an abomasal nematode of sheep in Burkina Faso. African Journal of Biotechnology, 8 (18): 4690-4695
Ahmed, M., Laing, M.D. and Nsahlai, I.V. (2012) In vitro anthelmintic activity of crude extracts of selected medicinal plants against Haemonchus contortus from sheep. Journal of Helminthology. 1-6
Akhtar, S., Dey, A. R., Hossain, S., Dey, T.R. and Begum, N. (2015) In vitro anthelmintic effect of some medicinal plants against Haemonchus contortus. J. Anim. Sci. Adv. 5(1): 1162-1170
Eugale, T., Tilahun, G., Gidey, M. and Mekonnen, Y. (2006)In vitro anthelmintic activities of four Ethiopian medicinal plants against Haemonchus contortus. Pharmacologyonline3: 153-165
Eugale, T., Tilahun, G., Dabella, A., Feleka, A. and Makonnen, E. (2007) Haemonchus contortus: in vitro and in vivo anthelmintic activity of aqueous and hydro-alcoholic extracts of Hedera helix. Experimental Parasitology., 116: 340-345
Ferreira, L.E., Castro, P.M.N., Chagas, A.C.S., Franca, S.C. and Beleboni, R.O. (2013) In vitro anthelmintic activity of aqueous leaf extract of Annona muricata L. (Annonaceae) against Haemonchus contortus from sheep. Augustein 1894 in Strongylusfilaria.Experimental Parasitology, 134: 327- 332
Guo, Z., Gonzalez, J.N. and Harnadez, J.N. (2016) Possible mechanisms of host resistance to Haemonchus contortus infection in sheep breeds native to Canary Islands. Scientific Reports. 6
Jabeen, N., Anwar, S., Mahmood, Q., Zia, M.A. and Murtaza, G. (2015) In vitro anthelmintic efficacy of native plants against Haemonchus contortus. Acta Poloniae Pharmaceutica.72(5): 1051-1055
Kornis, I.G, (1996) New Anthelmintics : Why so few? Proc. 41st Ann. Meet. Amer. Ass. Vet. Parasitologists. July 20-23,1996, Louisville, Kentucky, p.27.
Kuma, F., Birhanu and Hirpa, E. (2015) Advanced review on anthelmintic medicinal plants. Report and Opinion. 7(5): 7-16
Kumar, A. B. S., Lakshman, K., Jayaveera, K.N., Nandeesh, R., Manoj, B., and Ranganayakulu, D. (2010) Comparative in vitro anthelmintic activity of three plants from the Amaranthaceae family. Arch. Biol. Sci. Belgrade. 62(1): 185-189
Mali, R. and Mehta, A. (2008) A review on anthelmintic plants. Natural Product Radiance.7(5): 466-475
Mohammed, A., Wossene, A., Giday, M., Tilahun, G. and Kebede, N. (2013) In vitro anthelmintic activities of four medicinal plants against Haemonchus contortus.African Journal of Plant Science.7(8): 369-373
Qadir, S. Dixit, A. K. and Dixit, P. (2010) Use of medicinal plants to control Haemnchus contortus infection in small ruminants. Veterinary World. 3(11): 515-518
Rajeswari, V. D. (2014) Anthelmintic activity of plants: a review. Research Journal of Phytochemistry. 8(3): 57-63
Sanyal. P.K (1996) Parasitic gastroenteritis in ruminants in India. Proc. 8th Nat. Congr. Vet. Parasitol. pp. 7-16.
Singh, J. (2013) Anthelmintic activity of Acacia nilotica against Haemonchus contortus (Nematoda). Journal of Entomology and Zoology Studies.1(2): 47-53.
Singh, J. (2015) In vitro effect of neem leaf extract of the various organ-systems of Haemonchus contortus (Nematoda).Uttar Pradesh Journal of Zoology. 35(2): 161-168.
Urban, J., Kokoska, L. Langrova, I. and Matejkova, J. (2008) In vitro anthelmintic effects of medicinal plants used in Czeckh Republic. Pharmaceutical Biology. 46(10,11): 808-813
Vyas, B.N. and Mistry, K.B. (1996) Processing and Standardisation. In : Neem, N.S. Randhawa and B.S. Parmar (Eds.), New Age Intetnational Publications (P) Ltd., New Delhi and London, pp. 111-120.
Waller, P.J. and Thamasborg (2004) Nematode control in green ruminant production systems. Trends in Parasitology. 20(10): 493-497
Widiarso, B.P., Kurniasih, K, Prastowo, J. and Nurcahyo, W. (2018) Morphology and morphometry of Haemonchus contortus exposed to Gigantochloa apus crude aqueous extract. Veterinary World, 11: 921-925
Zenebe, S., Feyera, T. and Assefa, S. (2017) In vitro anthelmintic activity of crude extracts of aerial parts of Cissus quadrangularis L. and leaves of Schinus molle L. against Haemonchus contorus. BioMed Research International. pp1-6